Tryptic dissection and reconstitution of translocation activity for nascent presecretory proteins across microsomal membranes.
The ability of microsomal membranes to translocate nascent presecretory proteins across their lipid bilayer into the intravesicular space was investigated by using trypsin as a proteolytic probe. We found that under defined conditions trypsin is able to dissect the translocation activity of microsom...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1979
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383478/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/109833/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.4.1795 |
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