FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal

Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cy...

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Bibliografiske detaljer
Main Authors: Berger, Christian, Harzer, Heike, Burkard, Thomas R., Steinmann, Jonas, van der Horst, Suzanne, Laurenson, Anne-Sophie, Novatchkova, Maria, Reichert, Heinrich, Knoblich, Juergen A.
Format: Artigo
Sprog:Inglês
Udgivet: Cell Press 2012
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3828055/
https://ncbi.nlm.nih.gov/pubmed/22884370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2012.07.008
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