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Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei
A major obstacle to reproducible expression of recombinant transcripts lies in the epigenetic effects of the flanking chromatin following integration. We previously presented a strategy to overcome this problem in bloodstream form Trypanosoma brucei, using a reporter to identify a ribosomal-spacer l...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3828046/ https://ncbi.nlm.nih.gov/pubmed/18588918 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2008.05.006 |
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