A Simple Alkaline Method for Decellularizing Human Amniotic Membrane for Cell Culture

Human amniotic membrane is a standard substratum used to culture limbal epithelial stem cells for transplantation to patients with limbal stem cell deficiency. Various methods were developed to decellularize amniotic membrane, because denuded membrane is poorly immunogenic and better supports repopu...

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Main Authors: Saghizadeh, Mehrnoosh, Winkler, Michael A., Kramerov, Andrei A., Hemmati, David M., Ghiam, Chantelle A., Dimitrijevich, Slobodan D., Sareen, Dhruv, Ornelas, Loren, Ghiasi, Homayon, Brunken, William J., Maguen, Ezra, Rabinowitz, Yaron S., Svendsen, Clive N., Jirsova, Katerina, Ljubimov, Alexander V.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3827346/
https://ncbi.nlm.nih.gov/pubmed/24236148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0079632
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