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Evaluation of Reference Genes and Normalization Strategy for Quantitative Real-Time PCR in Human Pancreatic Carcinoma

Histologically verified pairs (n = 10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C(q) values correlated with the degree of RNA degradation. This correlation wa...

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Bibliografiske detaljer
Main Authors: Mohelnikova-Duchonova, Beatrice, Oliverius, Martin, Honsova, Eva, Soucek, Pavel
Format: Artigo
Sprog:Inglês
Udgivet: IOS Press 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3826910/
https://ncbi.nlm.nih.gov/pubmed/22377737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3233/DMA-2011-0875
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