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A novel electrochemical method for efficient reduction of disulfide bonds in peptides and proteins prior to MS detection

A novel electrochemical (EC) method for fast and efficient reduction of the disulfide bonds in proteins and peptides is presented. The method does not use any chemical agents and is purely instrumental. To demonstrate the performance of the EC reactor cell online with electrospray mass spectrometry,...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kraj, Agnieszka, Brouwer, Hendrik-Jan, Reinhoud, Nico, Chervet, Jean-Pierre
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer Berlin Heidelberg 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3826059/
https://ncbi.nlm.nih.gov/pubmed/24077854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-013-7374-3
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