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Measurement of the Change in Twist at a Helical Junction in RNA Using the Orientation Dependence of FRET
Indocarbocyanine fluorophores attached via the 5′ terminus of double-stranded nucleic acids have a strong propensity to stack onto the terminal basepair. We previously demonstrated that the efficiency of fluorescence resonance energy transfer between cyanine 3 and 5 terminally attached to duplex spe...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The Biophysical Society
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3824293/ https://ncbi.nlm.nih.gov/pubmed/24209863 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.09.042 |
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