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Measurement of the Change in Twist at a Helical Junction in RNA Using the Orientation Dependence of FRET

Indocarbocyanine fluorophores attached via the 5′ terminus of double-stranded nucleic acids have a strong propensity to stack onto the terminal basepair. We previously demonstrated that the efficiency of fluorescence resonance energy transfer between cyanine 3 and 5 terminally attached to duplex spe...

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Hlavní autoři: Fessl, Tomáš, Lilley, David M.J.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3824293/
https://ncbi.nlm.nih.gov/pubmed/24209863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.09.042
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