Generating In Vivo Cloning Vectors for Parallel Cloning of Large Gene Clusters by Homologous Recombination

A robust method for the in vivo cloning of large gene clusters was developed based on homologous recombination (HR), requiring only the transformation of PCR products into Escherichia coli cells harboring a receiver plasmid. Positive clones were selected by an acquired antibiotic resistance, which w...

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Detaylı Bibliyografya
Asıl Yazarlar: Lee, Jeongmin, Rha, Eugene, Yeom, Soo-Jin, Lee, Dae-Hee, Choi, Eui-Sung, Lee, Seung-Goo
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2013
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3823602/
https://ncbi.nlm.nih.gov/pubmed/24244585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0079979
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