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Generating In Vivo Cloning Vectors for Parallel Cloning of Large Gene Clusters by Homologous Recombination
A robust method for the in vivo cloning of large gene clusters was developed based on homologous recombination (HR), requiring only the transformation of PCR products into Escherichia coli cells harboring a receiver plasmid. Positive clones were selected by an acquired antibiotic resistance, which w...
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| Hlavní autoři: | , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Public Library of Science
2013
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3823602/ https://ncbi.nlm.nih.gov/pubmed/24244585 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0079979 |
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