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Generating In Vivo Cloning Vectors for Parallel Cloning of Large Gene Clusters by Homologous Recombination
A robust method for the in vivo cloning of large gene clusters was developed based on homologous recombination (HR), requiring only the transformation of PCR products into Escherichia coli cells harboring a receiver plasmid. Positive clones were selected by an acquired antibiotic resistance, which w...
সংরক্ষণ করুন:
| প্রধান লেখক: | , , , , , |
|---|---|
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
Public Library of Science
2013
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3823602/ https://ncbi.nlm.nih.gov/pubmed/24244585 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0079979 |
| ট্যাগগুলো: |
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