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Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions
Plasmid-based gene expression is a fundamental tool in the field of biotechnology. However, overexpression of genes of interest with multi-copy plasmids often causes detrimental effects on host cells. To overcome this problem, chromosomal integration of target genes has been used for decades; howeve...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5132264/ https://ncbi.nlm.nih.gov/pubmed/27907029 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0166890 |
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