Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions

Plasmid-based gene expression is a fundamental tool in the field of biotechnology. However, overexpression of genes of interest with multi-copy plasmids often causes detrimental effects on host cells. To overcome this problem, chromosomal integration of target genes has been used for decades; howeve...

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Publicat a:PLoS One
Autors principals: Yeom, Soo-Jin, Kim, Yu Jung, Lee, Jeongmin, Kwon, Kil Koang, Han, Gui Hwan, Kim, Haseong, Lee, Dae-Hee, Kim, Hak-Sung, Lee, Seung-Goo
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2016
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5132264/
https://ncbi.nlm.nih.gov/pubmed/27907029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0166890
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