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Development of Microsatellite Markers in Pungtungia herzi Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia
Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain react...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Molecular Diversity Preservation International (MDPI)
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3821594/ https://ncbi.nlm.nih.gov/pubmed/24084733 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms141019923 |
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