Development of Microsatellite Markers in Pungtungia herzi Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia

Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain react...

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Bibliografiske detaljer
Main Authors: Yun, Young-Eun, Yu, Jeong-Nam, Kim, Sang Ki, Hwang, Ui Wook, Kwak, Myounghai
Format: Artigo
Sprog:Inglês
Udgivet: Molecular Diversity Preservation International (MDPI) 2013
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3821594/
https://ncbi.nlm.nih.gov/pubmed/24084733
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms141019923
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