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Application of a qPCR Assay with Melting Curve Analysis for Detection and Differentiation of Protozoan Oocysts in Human Fecal Samples from Dominican Republic

A quantitative polymerase chain reaction assay with melt curve analysis (qPCR-MCA) was applied for the detection of protozoan oocysts in 501 human fecal samples collected in Dominican Republic. Samples were subjected to qPCR using universal coccidia primers targeting 18S rDNA to detect oocysts follo...

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Detalhes bibliográficos
Main Authors: Lalonde, Laura F., Reyes, Julissa, Gajadhar, Alvin A.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society of Tropical Medicine and Hygiene 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3820332/
https://ncbi.nlm.nih.gov/pubmed/24019437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.13-0106
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