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GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis

The Arabidopsis thaliana glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in H(2)O(2) homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX-6P1 and overexpressed in Escherichia coli. The GPX3 protein was purified to homogeneity in two chromatographic...

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Hlavní autoři: Li, Kun, Yang, Qingzhan, Wang, Wei, Zhao, Xiaoliang, Lou, Zhiyong
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3818038/
https://ncbi.nlm.nih.gov/pubmed/24192354
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113025566
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