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GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis
The Arabidopsis thaliana glutathione peroxidase 3 (GPX3) gene encodes a glutathione peroxidase with roles in H(2)O(2) homeostasis and signalling. The GPX3 gene sequence was cloned into pGEX-6P1 and overexpressed in Escherichia coli. The GPX3 protein was purified to homogeneity in two chromatographic...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
International Union of Crystallography
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3818038/ https://ncbi.nlm.nih.gov/pubmed/24192354 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113025566 |
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