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Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide–PMO (PPMO) examples ar...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Royal Society of Chemistry
2013
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3816334/ https://ncbi.nlm.nih.gov/pubmed/24064913 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3cc46067c |
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