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Labeling Live Cells by Copper-Catalyzed Alkyne-Azide Click Chemistry
The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, optimized for biological molecules in aqueous buffers, has been shown to rapidly label mammalian cells in culture with no loss in cell viability. Metabolic uptake and display of the azide derivative of N-acetylmannosamine developed by...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3014321/ https://ncbi.nlm.nih.gov/pubmed/20886827 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc100272z |
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