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Labeling Live Cells by Copper-Catalyzed Alkyne-Azide Click Chemistry

The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, optimized for biological molecules in aqueous buffers, has been shown to rapidly label mammalian cells in culture with no loss in cell viability. Metabolic uptake and display of the azide derivative of N-acetylmannosamine developed by...

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Detalhes bibliográficos
Main Authors: Hong, Vu, Steinmetz, Nicole F., Manchester, Marianne, Finn, M.G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3014321/
https://ncbi.nlm.nih.gov/pubmed/20886827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc100272z
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