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Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core
The purpose of this chapter is to provide practical chemical ligation procedures to prepare histone proteins suitable for the reconstitution of nucleosomes with specific posttranslational modifications in the nucleosome core. Detailed methods are described for the efficient preparation of semi synth...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3815671/ https://ncbi.nlm.nih.gov/pubmed/23381862 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-305-3_14 |
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