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Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination
Cas9 is an RNA-guided double-stranded DNA nuclease that participates in clustered regularly interspaced short palindromic repeats (CRISPR)-mediated adaptive immunity in prokaryotes. CRISPR–Cas9 has recently been used to generate insertion and deletion mutations in Caenorhabditis elegans, but not to...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3814388/ https://ncbi.nlm.nih.gov/pubmed/24013562 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt805 |
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