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Reversed-phase ion-pair liquid chromatography method for purification of duplex DNA with single base pair resolution
Obtaining quantities of highly pure duplex DNA is a bottleneck in the biophysical analysis of protein–DNA complexes. In traditional DNA purification methods, the individual cognate DNA strands are purified separately before annealing to form DNA duplexes. This approach works well for palindromic seq...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2013
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3814375/ https://ncbi.nlm.nih.gov/pubmed/24013567 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt815 |
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