Reversed-phase ion-pair liquid chromatography method for purification of duplex DNA with single base pair resolution

Obtaining quantities of highly pure duplex DNA is a bottleneck in the biophysical analysis of protein–DNA complexes. In traditional DNA purification methods, the individual cognate DNA strands are purified separately before annealing to form DNA duplexes. This approach works well for palindromic seq...

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Bibliografske podrobnosti
Main Authors: Wysoczynski, Christina L., Roemer, Sarah C., Dostal, Vishantie, Barkley, Robert M., Churchill, Mair E. A., Malarkey, Christopher S.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2013
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3814375/
https://ncbi.nlm.nih.gov/pubmed/24013567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt815
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