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Novel Method for Genomic Promoter Shuffling by Using Recyclable Cassettes
Genetic elements of interest can be introduced into the Saccharomyces cerevisiae genome via homologous recombination. The current method is to link such an element to a selectable marker gene to be integrated into the target locus. However, the marker gene in this method cannot be reused, which limi...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3811543/ https://ncbi.nlm.nih.gov/pubmed/24014535 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02159-13 |
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