Integrating Terminal Truncation and Oligopeptide Fusion for a Novel Protein Engineering Strategy To Improve Specific Activity and Catalytic Efficiency: Alkaline α-Amylase as a Case Study

In this work, we integrated terminal truncation and N-terminal oligopeptide fusion as a novel protein engineering strategy to improve specific activity and catalytic efficiency of alkaline α-amylase (AmyK) from Alkalimonas amylolytica. First, the C terminus or N terminus of AmyK was partially trunca...

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Bibliographic Details
Main Authors: Yang, Haiquan, Liu, Long, Shin, Hyun-dong, Chen, Rachel R., Li, Jianghua, Du, Guocheng, Chen, Jian
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2013
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3811186/
https://ncbi.nlm.nih.gov/pubmed/23956385
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02087-13
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