Use of virion DNA as a cloning vector for the construction of mutant and recombinant herpesviruses.
We have developed improved procedures for the isolation of deletion mutant, point mutant, and recombinant herpesvirus saimiri. These procedures take advantage of the absence of NotI and AscI restriction enzyme sites within the viral genome and use reporter genes for the identification of recombinant...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1996
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC38067/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8876145/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.21.11389 |
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