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Method for Isolation and Selection of Temperature-Sensitive Mutants of Herpes Simplex Virus

A method for induction and selection of temperature-sensitive mutants of herpes simplex virus is presented. After the infected cells were treated with 5-bromodeoxyuridine, the virus was extracted by repeated freezing and thawing and cloned in microcultures which were then incubated at permissive tem...

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Збережено в:
Бібліографічні деталі
Опубліковано в::Appl Microbiol
Автор: Manservigi, Roberto
Формат: Artigo
Мова:Inglês
Опубліковано: American Society for Microbiology (ASM) 1974
Предмети:
Онлайн доступ:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC380204/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4364609/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.27.6.1034-1040.1974
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