Method for Isolation and Selection of Temperature-Sensitive Mutants of Herpes Simplex Virus
A method for induction and selection of temperature-sensitive mutants of herpes simplex virus is presented. After the infected cells were treated with 5-bromodeoxyuridine, the virus was extracted by repeated freezing and thawing and cloned in microcultures which were then incubated at permissive tem...
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| Publicado no: | Appl Microbiol |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1974
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC380204/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4364609/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.27.6.1034-1040.1974 |
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