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Improved PCR Performance Using Template DNA from Formalin-Fixed and Paraffin-Embedded Tissues by Overcoming PCR Inhibition

Formalin-fixed and paraffin-embedded (FFPE) tissues represent a valuable source for biomarker studies and clinical routine diagnostics. However, they suffer from degradation of nucleic acids due to the fixation process. Since genetic and epigenetic studies usually require PCR amplification, this deg...

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Detalhes bibliográficos
Main Authors: Dietrich, Dimo, Uhl, Barbara, Sailer, Verena, Holmes, Emily Eva, Jung, Maria, Meller, Sebastian, Kristiansen, Glen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3796491/
https://ncbi.nlm.nih.gov/pubmed/24155973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0077771
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