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Additional TCRV beta primers and minor method modifications improve detection of clonal T-cell populations by RT-PCR.

The TCRV beta RT-PCR method for detection of clonal populations of T cells which we described previously could not detect clones that used certain variable (V) beta region families. V beta 2, 4, 8.3, and 18 had insufficient homology with the original consensus V region primer. Two new primers have b...

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Autors principals: Lynas, C., Howe, D.
Format: Artigo
Idioma:Inglês
Publicat: 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC379580/
https://ncbi.nlm.nih.gov/pubmed/9208815
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