Detection of clonal T cell populations by high resolution PCR using fluorescently labelled nucleotides; evaluation using conventional LIS-SSCP

Aims—To detect clonal T cell populations by high resolution polymerase chain reaction (PCR) using fluorescently labelled nucleotides and analysis on an ABI 377 DNA sequencer, and to evaluate this method using low ionic strength single strand conformation polymorphism (LIS-SSCP) analysis. Methods—DNA...

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Hauptverfasser: Wickham, C L, Lynas, C, Ellard, S
Format: Artigo
Sprache:Inglês
Veröffentlicht: British Medical Journal Publishing Group 2000
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Paper
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1186922/
https://ncbi.nlm.nih.gov/pubmed/10897335
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