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Detection of clonal T cell populations by high resolution PCR using fluorescently labelled nucleotides; evaluation using conventional LIS-SSCP

Aims—To detect clonal T cell populations by high resolution polymerase chain reaction (PCR) using fluorescently labelled nucleotides and analysis on an ABI 377 DNA sequencer, and to evaluate this method using low ionic strength single strand conformation polymorphism (LIS-SSCP) analysis. Methods—DNA...

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Détails bibliographiques
Auteurs principaux: Wickham, C L, Lynas, C, Ellard, S
Format: Artigo
Langue:Inglês
Publié: British Medical Journal Publishing Group 2000
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1186922/
https://ncbi.nlm.nih.gov/pubmed/10897335
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