Structural Insights into the Recovery of Aldolase Activity in N-Acetylneuraminic Acid Lyase by Replacement of the Catalytically Active Lysine with γ-Thialysine by Using a Chemical Mutagenesis Strategy

Chemical modification has been used to introduce the unnatural amino acid γ-thialysine in place of the catalytically important Lys165 in the enzyme N-acetylneuraminic acid lyase (NAL). The Staphylococcus aureus nanA gene, encoding NAL, was cloned and expressed in E. coli. The protein, purified in hi...

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Détails bibliographiques
Auteurs principaux: Timms, Nicole, Windle, Claire L, Polyakova, Anna, Ault, James R, Trinh, Chi H, Pearson, Arwen R, Nelson, Adam, Berry, Alan
Format: Artigo
Langue:Inglês
Publié: WILEY-VCH Verlag 2013
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Full Papers
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792637/
https://ncbi.nlm.nih.gov/pubmed/23418011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201200714
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