The Mechanism of the Amidases: MUTATING THE GLUTAMATE ADJACENT TO THE CATALYTIC TRIAD INACTIVATES THE ENZYME DUE TO SUBSTRATE MISPOSITIONING

All known nitrilase superfamily amidase and carbamoylase structures have an additional glutamate that is hydrogen bonded to the catalytic lysine in addition to the Glu, Lys, Cys “catalytic triad.” In the amidase from Geobacillus pallidus, mutating this glutamate (Glu-142) to a leucine or aspartate r...

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Detalhes bibliográficos
Main Authors: Weber, Brandon W., Kimani, Serah W., Varsani, Arvind, Cowan, Donald A., Hunter, Roger, Venter, Gerhard A., Gumbart, James C., Sewell, B. Trevor
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2013
Assuntos:
Enzymology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3789952/
https://ncbi.nlm.nih.gov/pubmed/23946488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.503284
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