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Efficient Single-Cell Transgene Induction in Caenorhabditis elegans Using a Pulsed Infrared Laser
The coupling of transgenes to heat shock promoters is a widely applied method for regulating gene expression. In C. elegans, gene induction can be controlled temporally through timing of heat shock and spatially via targeted rescue in heat shock mutants. Here, we present a method for evoking gene ex...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Genetics Society of America
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3789807/ https://ncbi.nlm.nih.gov/pubmed/23979939 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.113.007682 |
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