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Efficient Single-Cell Transgene Induction in Caenorhabditis elegans Using a Pulsed Infrared Laser
The coupling of transgenes to heat shock promoters is a widely applied method for regulating gene expression. In C. elegans, gene induction can be controlled temporally through timing of heat shock and spatially via targeted rescue in heat shock mutants. Here, we present a method for evoking gene ex...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3789807/ https://ncbi.nlm.nih.gov/pubmed/23979939 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.113.007682 |
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