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A new strategy for gene targeting and functional proteomics using the DT40 cell line

DT40 cells derived from chicken B lymphocytes exhibit exceptionally high homologous recombination rates. Therefore, they can be used as a convenient tool and model for gene targeting experiments. However, lack of efficient cloning strategies, protein purification protocols and a well annotated prote...

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Detalhes bibliográficos
Main Authors: Orlowska, Kinga P., Klosowska, Kamila, Szczesny, Roman J., Cysewski, Dominik, Krawczyk, Pawel S., Dziembowski, Andrzej
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3783193/
https://ncbi.nlm.nih.gov/pubmed/23892402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt650
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