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Single DNA Molecule Patterning for High-Throughput Epigenetic Mapping

We present a method for profiling the 5-methyl cytosine distribution on single DNA molecules. Our method combines soft-lithography and molecular elongation to form ordered arrays of more than 250,000 individual DNA molecules immobilized on a solid substrate. The methylation state of the DNA is detec...

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Detalhes bibliográficos
Main Authors: Cerf, Aline, Cipriany, Benjamin R., Benítez, Jaime J., Craighead, Harold G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3773698/
https://ncbi.nlm.nih.gov/pubmed/21981444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac202506j
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