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A mutational analysis of 48G7 reveals that somatic hypermutation affects both antibody stability and binding affinity

The monoclonal antibody 48G7 differs from its germline precursor by ten somatic mutations, a number of which appear to be functionally silent. We analyzed the effects of individual somatic mutations and combinations thereof on both antibody binding affinity and thermal stability. Individual somatic...

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Detalhes bibliográficos
Main Authors: Sun, Sophie B., Sen, Shiladitya, Kim, Nam Jung, Magliery, Thomas J., Schultz, Peter G., Wang, Feng
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3773045/
https://ncbi.nlm.nih.gov/pubmed/23795814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja402927u
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