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High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
CRISPR RNA-guided endonucleases (RGENs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell-based reporter assay to characterize off-target cleavage of Cas9-based RGENs. We find that single and double mismatches are tolerated to varying degrees depen...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3773023/ https://ncbi.nlm.nih.gov/pubmed/23792628 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2623 |
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