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Rapid genotyping using pyrene−perylene locked nucleic acid complexes

We have developed an assay for single strand DNA and RNA detection which is based on novel pyrene−perylene FRET pairs attached to short LNA/DNA probes. The assay is based on ratiometric emission upon binding of target DNA/RNA by three combinations of fluorescent LNA/DNA reporter strands. Specific ge...

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Main Authors: Kumar, T. Santhosh, Myznikova, Anna, Samokhina, Evgeniya, Astakhova, Irina Kira
格式: Artigo
語言:Inglês
出版: Landes Bioscience 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3771999/
https://ncbi.nlm.nih.gov/pubmed/24044052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/adna.25903
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