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Rapid genotyping using pyrene−perylene locked nucleic acid complexes

We have developed an assay for single strand DNA and RNA detection which is based on novel pyrene−perylene FRET pairs attached to short LNA/DNA probes. The assay is based on ratiometric emission upon binding of target DNA/RNA by three combinations of fluorescent LNA/DNA reporter strands. Specific ge...

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Detalhes bibliográficos
Main Authors: Kumar, T. Santhosh, Myznikova, Anna, Samokhina, Evgeniya, Astakhova, Irina Kira
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3771999/
https://ncbi.nlm.nih.gov/pubmed/24044052
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/adna.25903
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