Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that...

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Hlavní autoři: Miyata, Marcelo, Santos, Adolfo Carlos Barreto, Mendes, Natália Helena, Cunha, Eunice Atsuko, de Melo, Fernando Augusto Fiúza, Leite, Clarice Queico Fujimura
Médium: Artigo
Jazyk:Inglês
Vydáno: Sociedade Brasileira de Microbiologia 2011
Témata:
Genetics and Molecular Microbiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3769851/
https://ncbi.nlm.nih.gov/pubmed/24031692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-838220110002000045
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