Quantification of Anti-Aggregation Activity of Chaperones: A Test-System Based on Dithiothreitol-Induced Aggregation of Bovine Serum Albumin

The methodology for quantification of the anti-aggregation activity of protein and chemical chaperones has been elaborated. The applicability of this methodology was demonstrated using a test-system based on dithiothreitol-induced aggregation of bovine serum albumin at 45°C as an example. Methods fo...

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Main Authors: Borzova, Vera A., Markossian, Kira A., Kara, Dmitriy A., Chebotareva, Natalia A., Makeeva, Valentina F., Poliansky, Nikolay B., Muranov, Konstantin O., Kurganov, Boris I.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2013
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3769246/
https://ncbi.nlm.nih.gov/pubmed/24058554
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0074367
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