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A novel method that improves sensitivity of protein detection in PAGE and western blot

We have developed a simple and inexpensive method that improves sensitivity of protein and antigen detection in standard PAGE procedures. Our technique uses a sample microloader device with a funnel-like structure, filled with a 4% stacking gel. When attach to the top of a polyacrylamide slab gel th...

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Autori principali: Vallejo-Illarramendi, Ainara, Marciano, Denise K., Reichardt, Louis F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3768259/
https://ncbi.nlm.nih.gov/pubmed/23400834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201200534
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