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Prolonged Incubation and Stacked Film Exposure Improve Sensitivity in Western Blotting

INTRODUCTION: Western blotting is a basic technique for protein detection. For proteins of less abundance or antibodies of poorer quality, an increased sensitivity is often desired. Although it is commonly known that higher concentrations of antibodies and prolonged film exposure times will help imp...

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Autori principali: Luo, Haitao, Rankin, Gary O., Straley, Shannon, Chen, Yi Charlie
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3204169/
https://ncbi.nlm.nih.gov/pubmed/21741488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.vascn.2011.06.001
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