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Dual Role of φ29 DNA Polymerase Lys529 in Stabilisation of the DNA Priming-Terminus and the Terminal Protein-Priming Residue at the Polymerisation Site

Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3′ terminal phosphodiester bond. In this paper, site-directed mutants at this Lys residue were us...

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Bibliografski detalji
Glavni autori: del Prado, Alicia, Lázaro, José M., Villar, Laurentino, Salas, Margarita, de Vega, Miguel
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3762793/
https://ncbi.nlm.nih.gov/pubmed/24023769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0072765
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