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Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation

In recent years, stable isotope labeling by amino acids in cell culture (SILAC) has become increasingly popular as a quantitative proteomic method. In SILAC experiments, proteins are metabolically labeled by culturing cells in media containing normal and heavy isotope amino acids. This makes protein...

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Autores principales: Zhang, Guoan, Neubert, Thomas A.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2009
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3757925/
https://ncbi.nlm.nih.gov/pubmed/19241007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-834-8_7
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