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Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation

In recent years, stable isotope labeling by amino acids in cell culture (SILAC) has become increasingly popular as a quantitative proteomic method. In SILAC experiments, proteins are metabolically labeled by culturing cells in media containing normal and heavy isotope amino acids. This makes protein...

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Detalhes bibliográficos
Main Authors: Zhang, Guoan, Neubert, Thomas A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3757925/
https://ncbi.nlm.nih.gov/pubmed/19241007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-834-8_7
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