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Isolation and in vitro culture of rare cancer stem cells from patient-derived xenografts of pancreatic ductal adenocarcinoma

Described is the construction of a large array of releasable microstructures (micropallets) along with screening and isolation protocols for sorting rare, approximately 1 in 10,000, cancer stem cells (CSCs) from a heterogeneous cell population. A 10.1 × 7.1 cm array of micropallets (50 × 50 × 75 μm...

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Detalhes bibliográficos
Main Authors: Gach, Philip C., Attayek, Peter J., Herrera, Gabriela, Yeh, Jen Jen, Allbritton, Nancy L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3754897/
https://ncbi.nlm.nih.gov/pubmed/23815678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac401165s
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