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Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli

The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase...

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Autors principals: Aboulnaga, El-Hussiny, Pinkenburg, Olaf, Schiffels, Johannes, El-Refai, Ahmed, Buckel, Wolfgang, Selmer, Thorsten
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3754583/
https://ncbi.nlm.nih.gov/pubmed/23772070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00321-13
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