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An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli

Expression of multiple heterologous genes in a dedicated host is a prerequisite for approaches in synthetic biology, spanning from the production of recombinant multiprotein complexes to the transfer of tailor-made metabolic pathways. Such attempts are often exacerbated, due in most cases to a lack...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Schiffels, Johannes, Pinkenburg, Olaf, Schelden, Maximilian, Aboulnaga, El-Hussiny A. A., Baumann, Marcus E. M., Selmer, Thorsten
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3702609/
https://ncbi.nlm.nih.gov/pubmed/23861944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0068812
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