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Platform Engineering of Corynebacterium glutamicum with Reduced Pyruvate Dehydrogenase Complex Activity for Improved Production of l-Lysine, l-Valine, and 2-Ketoisovalerate

Exchange of the native Corynebacterium glutamicum promoter of the aceE gene, encoding the E1p subunit of the pyruvate dehydrogenase complex (PDHC), with mutated dapA promoter variants led to a series of C. glutamicum strains with gradually reduced growth rates and PDHC activities. Upon overexpressio...

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Bibliografiske detaljer
Main Authors: Buchholz, Jens, Schwentner, Andreas, Brunnenkan, Britta, Gabris, Christina, Grimm, Simon, Gerstmeir, Robert, Takors, Ralf, Eikmanns, Bernhard J., Blombach, Bastian
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3754147/
https://ncbi.nlm.nih.gov/pubmed/23835179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01741-13
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