Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells

Recently, we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a detectable fluorescent signal in living cells that express the target RNA. Here, we show that RBMBs can also be used to image single RNA transcripts in living cells, when the target RNA is...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Zhang, Xuemei, Song, Yang, Shah, Akash Y., Lekova, Virzhiniya, Raj, Arjun, Huang, Ling, Behlke, Mark A., Tsourkas, Andrew
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: Oxford University Press 2013
Θέματα:
Methods Online
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3753654/
https://ncbi.nlm.nih.gov/pubmed/23814183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt561
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