Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells

Recently, we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a detectable fluorescent signal in living cells that express the target RNA. Here, we show that RBMBs can also be used to image single RNA transcripts in living cells, when the target RNA is...

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Bibliografske podrobnosti
Main Authors: Zhang, Xuemei, Song, Yang, Shah, Akash Y., Lekova, Virzhiniya, Raj, Arjun, Huang, Ling, Behlke, Mark A., Tsourkas, Andrew
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2013
Teme:
Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3753654/
https://ncbi.nlm.nih.gov/pubmed/23814183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt561
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